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The vials were gently shaken for 30 s, and the concentration of CH 4 and CO 2 in the headspace was measured by GC/FID (as above) and then resampled over time (as above). The vials were incubated on rollers at 10 °C to mimic average river water temperature (Mackey & Berrie, 1991 ), and the change in total CH 4 (headspace and water phase) was used to calculate the rate of CH 4 oxidation.

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